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Mapping of QTLs and gene identification for fruit development, time to flowering and biotic stresses by performing genetic and transcriptomic approaches.
Development of a reverse genetic tool, TILLING which is based on production of common bean EMS-mutagenised plant collection and rapid systematic identification of mutations in target sequences. Breeding genetic material for dry and snap bean, popped-snack beans, tomato, pepper and other legume varieties. |
Common bean landrace and wild type germplasm collection
A wide collection of common bean landraces and wild types from different geographic origins conserved in the germplasm bank.
Characterized for a wide range of fruit development, time to flowering, quality and biotic resistance phenotypic traits. Evaluated on a broad range of environments and photoperiod conditions. Genotyped for a different set of molecular markers (SSRs, SNPs, etc.) covering linkage groups of common bean genome. Databases to manage and integrate the data from both the phenotype and genotype recordings. |
Large EMS common bean mutant collection
The production and seed and leaf management of about 5.000 common bean EMS mutants.
Over 2.500 M2 families screened, and advanced to M3. The development of HRM tools for rapid and systematic identification of mutations. The selection of target genes of agronomic importance to be TILLED in the crop. Databases to manage and integrate the data from both the phenotype recordings and TILLING platform. |
Mapping common bean population collection
Recombinant Inbreed Line populations developed for different traits.
SNP sets by re-sequencing breeding lines, which will be used to saturate genetic maps and to obtain molecular markers for marker-assisted breeding of qualitative and quantitative traits. Genetic linkage map consisted of 229 loci (86 AFLPs, 98 SSRs, 42 SNPs, 2 SCARs, and P locus) and distributed on 11 Linkage Groups, spanned 858.4 cM, with an average distance of 3.7 cM between adjacent markers. |