EMS mutant collection
By mutagenizing with different ethyl methyl sulfone (EMS) concentrations, we have developed a permanent collection of 2934 M2 independent mutant lines in the reference genotype Bolita of common bean. The frequency of mutations per line was maximized by using M1 plants with low but sufficient seed fertility. Phenotype scoring was performed at different developmental stages from seed germination through fruit maturation and seed harvest. We obtained a number of mutant lines showing inheritable morphological changes in plant architecture, leaves, floral organs, fruits and other traits through M1, M2 and M3 generations.
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